Chymotrypsin
Source: Bovine (Porcine) pancreasChymotrypsin which is the endopeptidase extracted and separated from the fresh pancreas of bovine (or porcine).
Primary structure and spatial structure of α- Chymotrypsin have been clearly explained, which is a single peptide chain composed of 245 amino acid residues, with 5 pairs of disulfide linkage in the molecule. It is white and light yellow crystal or amorphous powder, and easily soluble in the water, but insoluble in organic solvent. Relative molecular mass is 24000, and the optimum PH is 8~9. It is stable at a dry state, but will be quickly deactivated in water solution, most stable in the water solution with PH3~4. When it acts on the protein, it will hydrolyze the peptide bond formed by the carboxyl of L-tyrosine and L-phenylalanine in priority.
The high purity Chymotrypsin of Beijing Geyuantianrun Bio-tech Co., Ltd.. is purified by re-crystallization, and then by Ion Exchange Chromatography and ultra-filtration.
Application
--In the clinical, it always used to general trauma, wound healing that after surgery, anti-inflammatory, prevent local edema, hematocele, sprain hematoma, partial swelling that after breast surgery, tympanitis, rhinitis and so on. Use for the removal of cataract, Loose the lashes muscle ligament, reduce the cyst rupture and the hurt of retina.Besides, the combination use with the Trypsin and used as a broad-spectrum anti-inflammatory drugs.
--as the auxiliary medicine of antibiotic and antifungal drugs,it is widely used in the field of animal medicine and animal health industry.
Specification
| Items | Specification | Method |
| Appearance | White or almost white lyophilized powder | USP33 |
| Identification | No purple color develops within 3 minutes | USP33 |
| Loss on drying | No more than 5.0% | USP33 |
| Residue on Ignition | No more than 2.5% | USP33 |
| Microbial limits: Pseudomonas aeruginosa Salmonella Staphylococcus aureus |
Absent | USP33 |
| Trypsin | No more than 1% | USP33 |
| Assay | Chymotrypsin≥1000 USPunits/mg powder | USP33 |
| Items | Specification | Standard |
| Description | White crystalline lyophilized powder | EP9.0 |
| Identification | 1) A purple colour develops 2)No purple colour develops within 3 min of mixing |
EP9.0 |
| Appearance of solution | Solution S is not more opalescent than reference suspension Ⅱ | EP9.0 |
| Absorbance | 1) 281nm : 18.5 ~ 22.5 2) 250nm : not more than 8.0 |
EP9.0 |
| PH | 3.0-5.0 | EP9.0 |
| Histamine | Not more than 1µg/5.0µkatal | EP9.0 |
| Loss on drying | Not more than 5% | EP9.0 |
| Limit of trypsin | Not more than 1% | EP9.0 |
| Microbial limits: Pseudomonas aeruginosa Salmonella |
Absent | EP9.0 |
| Assay | Not less than 5.0 ukatal/mg, calculated on the dried basis | EP9.0 |
Sealed, Dark, at temperature 2-8℃